抗草甘膦转基因大豆对根际土壤细菌多样性的影响

被引:7
作者
李刚
赵建宁
杨殿林
机构
[1] 农业部环境保护科研监测所农业部转基因生物生态环境安全监督检验测试中心
关键词
抗草甘膦转基因大豆; 根际土壤; 细菌多样性; DGGE-cloning测序技术;
D O I
暂无
中图分类号
S565.1 [大豆];
学科分类号
摘要
采用DGGE-cloning测序技术研究抗草甘膦转基因大豆在生长期内对根际土壤细菌多样性的影响。结果分析表明,不同生长期内抗草甘膦转基因大豆与非转基因大豆根际土壤细菌16S rDNADGGE指纹图谱谱形相似,仅在出苗期和鼓粒期出现两条差异带,分别属于芽单胞菌门(UnculturedGemmatimonadetes bacterium clone,缺失)和壁厚菌门(Uncultured Firmicutes bacterium cloneGASP-KC3W1H04,增加);不同生长期内抗草甘膦转基因大豆与非转基因大豆根际土壤细菌16SrDNA DGGE指纹图谱的多样性指数和均匀度指数无显著差异。因此,抗草甘膦转基因大豆对土壤细菌多样性无显著影响。
引用
收藏
页码:100 / 104
页数:5
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