一氧化碳释放分子2对休克大鼠肠黏膜上皮屏障的保护作用

被引：7

作者：

王晓红 ^{[1
]}

刘芳 ^{[2
]}

张胜 ^{[2
]}

马希刚 ^{[1
]}

机构：

[1] 宁夏医科大学总医院重症医学科,银川

[2] 宁夏医科大学,银川

来源：

中华急诊医学杂志 | 2019年 / 28卷 / 01期

关键词：

一氧化碳释放分子2; 休克; 肠; 紧密连接; 炎症;

D O I：

暂无

中图分类号：

R459.7 [急症、急救处理];

学科分类号：

100231 [临床病理学];

摘要：

目的探讨一氧化碳释放分子-2(CORM-2)对休克肠屏障损伤的保护作用。方法采用Wiggers改良法制备失血性休克大鼠模型, 32只SD大鼠随机(随机数字法)分为4组:假手术组、休克组、CORM-2组及iCORM-2组(灭活型CORM-2组), 每组各8只。CORM-2组及iCORM-2组干预药物均为5 mg/kg制作休克模型前1 h腹腔注射;各组于术后或制作休克模型24 h后留取回肠组织观察病理形态及超微结构;Western-blot检测回肠组织ZO-1、Occludin、Claudin3蛋白表达;ELISA法测定血D-乳酸及TNF-α含量。各组间采用单因素方差分析, 组间两两比较采用LSD-t检验,方差不齐时采用Kruskal Wallis秩和检验, 以P<0.05为差异有统计学意义。结果 (1)休克组大鼠回肠黏膜病理形态和超微结构损伤明显。CORM-2可明显改善休克大鼠肠黏膜病理形态和超微结构完整性。(2)与假手术组比较, 休克组和iCORM-2组的Claudin3、Occludin、ZO-1蛋白表达均降低(均P<0.05), CORM-2组仅Claudin3、ZO-1蛋白表达降低(均P<0.05)。CORM-2组上述蛋白表达均高于休克组及iCORM-2组(均P<0.05)。(3)与假手术组比较, 休克组和iCORM-2组血D-乳酸及TNF-α水平明显升高[D-乳酸(ng/mL)分别为139.49±28.83, 135.75±25.19vs 65.09±33.16;TNF-α(pg/mL)分别为358.60(314.18, 395.73), 312.25(275.75, 345.40)vs 65.85(47.82, 84.32), 均P<0.05]。CORM-2组血D-乳酸及TNF-α水平有升高趋势, 但差异无统计学意义[D-乳酸(ng/mL)75.65±34.14vs 65.09±33.16;TNF-α(pg/mL)104.00(93.10, 131.95)vs 65.85(47.82, 84.32), 均P>0.05]。CORM-2组血D-乳酸及TNF-α水平均低于休克组和iCORM-2组(均P<0.05)。结论 CORM-2可减轻休克肠屏障损伤, 并上调肠上皮紧密连接蛋白表达, 其抗炎效应可能是其发挥保护作用的机制之一。

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