金魁猕猴桃RT-qPCR内参基因的筛选附视频

被引:6
作者
张计育
黄胜男
王涛
潘德林
翟敏
郭忠仁
机构
[1] 江苏省中国科学院植物研究所
关键词
猕猴桃; 内参基因; 实时荧光定量PCR;
D O I
10.15955/j.issn1000-3924.2018.01.16
中图分类号
S663.4 [猕猴桃];
学科分类号
摘要
以'金魁'猕猴桃(Actinidia deliciosa)根、茎、叶、花瓣、花萼、雌蕊、子房、幼果为材料,应用实时荧光定量PCR(RT-qPCR)技术分析了ACT、CYP2、RP2、GAPDH、TUB和TUA 6个常用内参基因在猕猴桃不同器官组织中的表达情况,并利用GeNorm、NormFinder和BestKeeper软件对候选内参基因的稳定性进行了评价。结果表明:ACT和TUA基因在各组织中的表达量差异较小,表达相对稳定;在利用RT-qPCR分析比较猕猴桃不同器官组织中的基因表达差异时,可选择ACT作为内参基因。
引用
收藏
页码:84 / 88
页数:5
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