双重实时荧光PCR法测定食品中大豆、小麦过敏原

被引：4

作者：

金萍

丁洪流

张敏

金晓红

机构：

[1] 苏州市产品质量监督检验院

来源：

食品与机械 | 2022年 / 38卷 / 09期

关键词：

实时荧光聚合酶链反应; 食品过敏原; 大豆; 小麦;

D O I：

10.13652/j.spjx.1003.5788.2022.90116

中图分类号：

TS207.3 [食品分析与检验];

学科分类号：

摘要：

目的:为食品监管部门有效检测食品中大豆、小麦过敏原提供技术支撑。方法:分别依据小麦醇溶蛋白基因及大豆Lectin基因为模板设计并创建TaqMan探针双重荧光PCR方法，大豆Lectin基因检测采用FAM标记，小麦醇溶蛋白基因检测采用HEX标记，同时以真核生物18S rRNA作为内参基因确保检测体系的有效性。结果:所创建的实时多重TaqMan探针PCR体系对大豆、小麦过敏原之外的物种成分无荧光扩增；大豆、小麦混合样品的检出限均能达到0.01%(质量分数)。结论:所创建的实时多重TaqMan探针PCR体系针对大豆、小麦过敏原有高特异性，可用于食品中过敏原大豆、小麦成分的同步快速检测。

引用

页码：59 / 63+102 +102

页数：6

共 26 条

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