大鼠脂多糖结合蛋白的分离纯化及对脂多糖的调节作用

被引:3
作者
侯一峰
毛宝龄
钱桂生
徐剑铖
机构
[1] 第三军医大学附属新桥医院呼吸病研究所!重庆,第三军医大学附属新桥医院呼吸病研究所!重庆,第三军医大学附属新桥医院呼吸病研究所!重庆,第三军医大学附属新桥医院呼吸病研究所!重庆
关键词
脂多糖结合蛋白; 脂多糖; 分离纯化; TNF-α;
D O I
10.13865/j.cnki.cjbmb.2001.05.024
中图分类号
Q513 [结合蛋白质]; Q538 [结合糖];
学科分类号
071010 ; 081704 ;
摘要
经硫酸铵沉淀、Bio Rex70树脂的阳离子交换层析和MonoQ预装柱的阴离子交换层析 ,从大鼠急性期血清中分离纯化了脂多糖结合蛋白 .SDS PAGE为单一条带 ,分子量 60kD ,所纯化的大鼠脂多糖结合蛋白可明显增强脂多糖与单核巨噬细胞的结合 .脂多糖结合蛋白对脂多糖诱导肺泡巨噬细胞中肿瘤坏死因子α(TNF α)mRNA表达的调节作用具有明显的剂量依赖性 .
引用
收藏
页码:647 / 650
页数:4
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