川西北高寒草甸土壤微生物功能多样性对磷(P)添加的响应

被引:8
作者
字洪标 [1 ]
代迪 [2 ]
胡雷 [2 ]
阿的鲁骥 [1 ]
王长庭 [2 ]
机构
[1] 西南民族大学青藏高原研究院
[2] 西南民族大学生命科学与技术学院
关键词
高寒草甸; 土壤微生物群落; Biolog-ECO; P肥; 施肥梯度;
D O I
10.19336/j.cnki.trtb.2017.03.20
中图分类号
S812.2 [草原土壤学];
学科分类号
0713 ;
摘要
研究高寒草甸添加不同磷(P)肥梯度对土壤养分状况与土壤微生物的影响,探明土壤养分变化和微生物功能多样性的差异及其互作关系。采用常规实验室分析和Biolog-ECO生态板法,研究了0 g m-2(CK),10 g m-2(P10),20 g m-2(P20),30 g m-2(P30)4个施P肥水平下土壤养分及土壤微生物功能多样性的变化规律。结果表明:P肥显著增加高寒草甸的速效养分、全磷和全钾的含量,但对有机质、全氮和pH无显著影响。AWCD值在表层(0~10 cm)无显著变化,但深层(10~20 cm)则为P20>P30>P10>CK。土壤微生物多样性在表层随施肥量增加而降低,而深层随施肥量增加而升高。主成分分析表明,氨基酸类、胺类、酯类是土壤微生物利用的主要碳源类型,其中在表层糖类和酯类随施肥量增加而降低,而深层碳源利用均在P20处理下显著提高。冗余分析表明,土壤速效氮、速效磷和速效钾含量是影响高寒草甸土壤微生物功能多样性和代谢活性的主要因子。因此,在退化高寒草甸适量添加P肥有利于生态系统的恢复重建。
引用
收藏
页码:647 / 655
页数:9
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