CRISPR/Cas9系统在植物基因功能研究中的应用进展

被引：27

作者：

霍晋彦

李姣

荆雅峰

冯宝民

于宗霞

机构：

[1] 大连大学生命科学与技术学院

来源：

植物生理学报 | 2019年 / 55卷 / 03期

关键词：

CRISPR/Cas9; 基因编辑; 植物基因功能;

D O I：

10.13592/j.cnki.ppj.2019.0037

中图分类号：

Q943.2 [植物基因工程];

学科分类号：

071007 [遗传学];

摘要：

随着基因组时代到来,涌现出海量基因数据,仅依靠传统过量表达、RNA干扰(RNAi)等手段难以满足大量基因功能分析的需求。依赖于微生物的CRISPR/Cas系统依靠其操作简便、基因编辑效率高、适用范围广的特点,迅速成为研究植物、动物、微生物基因功能的重要手段。本文主要介绍了CRISPR/Cas系统的产生、发展和作用机理,并重点介绍了CRISPR/Cas9在植物基因功能研究方面的应用,从而为该系统的进一步优化和多元化应用提供参考。

引用

收藏

页码：241 / 246

页数：6

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