敲除巨噬细胞对胆管结扎小鼠胆汁淤积的干预作用

被引：1

作者：

田心蓓

陈珊珊

王伟鹏

吴博

朱景

周莹

肖永陶

机构：

[1] 上海交通大学医学院附属新华医院上海市儿科医学研究所上海市小儿消化与营养重点实验室

来源：

临床儿科杂志 | 2021年 / 39卷 / 07期

关键词：

巨噬细胞; 胆汁酸; 肠道菌群; 胆道闭锁; 小鼠;

D O I：

暂无

中图分类号：

R725.7 [小儿消化系及腹部疾病];

学科分类号：

100201 [内科学];

摘要：

目的探讨巨噬细胞对胆汁酸代谢的影响。方法以结扎7周龄雄性CD11b-DTR小鼠胆总管方法建立胆汁淤积模型,2周后测定肝脏及结肠内容物中胆汁酸谱及肠道菌群组成。将28只小鼠分为假手术(Sham)组、假手术干预(DT)组、胆管结扎(BDL)组、胆管结扎干预(BDL+DT)组。结果与Sham组相比,BDL组肝脏中初级胆汁酸及总胆汁酸含量均显著增加,BDL+DT组肝脏中非结合初级胆汁酸及总胆汁酸含量则较BDL组明显减少,差异有统计学意义(P均<0.05)。在结肠内容物中,BDL+DT组较BDL组结合初级胆汁酸含量降低,差异有统计学意义(P<0.05)。BDL+DT组肝内胆汁酸合成关键酶cyp7a1表达量较BDL组降低,差异有统计学意义(P<0.05)。与Sham组相比,BDL组肠道Prevotella的属水平相对丰度增加,而BDL+DT组Escherichia、Clostridium和Segmented filamentous bacteria(SFB)较BDL组属水平相对丰度增加,差异均有统计学意义(P均<0.05)。结论巨噬细胞条件性敲除可通过改变肠道菌群结构干预胆汁淤积。

引用

页码：520 / 524

页数：5

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