Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells

被引：982

作者：

Atarashi, Koji ^{[2
,4
]}

Tanoue, Takeshi ^{[2
]}

Ando, Minoru ^{[1
]}

Kamada, Nobuhiko ^{[5
]}

Nagano, Yuji ^{[2
]}

Narushima, Seiko ^{[2
]}

Suda, Wataru ^{[4
,8
]}

Imaoka, Akemi ^{[1
]}

Setoyama, Hiromi ^{[1
]}

Nagamori, Takashi ^{[1
,9
]}

Ishikawa, Eiji ^{[1
]}

Shima, Tatsuichiro ^{[1
]}

Hara, Taeko ^{[1
]}

Kado, Shoichi ^{[1
]}

Jinnohara, Toshi ^{[2
]}

Ohno, Hiroshi ^{[2
]}

Kondo, Takashi ^{[2
]}

Toyooka, Kiminori ^{[3
]}

Watanabe, Eiichiro ^{[2
]}

Yokoyama, Shin-ichiro ^{[11
]}

Tokoro, Shunji ^{[11
]}

Mori, Hiroshi ^{[11
]}

Noguchi, Yurika ^{[9
]}

Morita, Hidetoshi ^{[9
,13
]}

Ivanov, Ivaylo I. ^{[10
]}

Sugiyama, Tsuyoshi ^{[11
]}

Nunez, Gabriel ^{[6
,7
]}

Camp, J. Gray ^{[12
]}

Hattori, Masahira ^{[4
,8
]}

Umesaki, Yoshinori ^{[1
]}

Honda, Kenya ^{[2
,4
,13
]}

机构：

[1] Yakult Cent Inst, Kunitachi, Tokyo 1868650, Japan

[2] RIKEN Ctr Integrat Med Sci IMS, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

[3] CSRS, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

[4] Keio Univ, Sch Med, Dept Microbiol & Immunol, Shinjuku Ku, Tokyo 1608582, Japan

[5] Univ Michigan, Sch Med, Dept Internal Med, Div Gastroenterol, Ann Arbor, MI 48109 USA

[6] Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI 48109 USA

[7] Univ Michigan, Sch Med, Ctr Comprehens Canc, Ann Arbor, MI 48109 USA

[8] Univ Tokyo, Grad Sch Frontier Sci, Kashiwa, Chiba 2778561, Japan

[9] Azabu Univ, Sch Vet Med, Sagamihara, Kanagawa 2525201, Japan

[10] Columbia Univ, Med Ctr, Dept Microbiol & Immunol, New York, NY 10032 USA

[11] Gifu Pharmaceut Univ, Dept Biopharmaceut Sci, Lab Microbiol & Immunol, Gifu 5011196, Japan

[12] Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, D-04103 Leipzig, Germany

[13] JST, CREST, Kawaguchi, Saitama 3320012, Japan

来源：

CELL | 2015年 / 163卷 / 02期

基金：

日本学术振兴会;

关键词：

SEGMENTED FILAMENTOUS BACTERIA; ESCHERICHIA-COLI O157-H7; IMMUNE-SYSTEM; T(H)17 CELLS; DIFFERENTIATION; HOST; COLONIZATION; ATTACHMENT; MATURATION; SEQUENCES;

D O I：

10.1016/j.cell.2015.08.058

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Intestinal Th17 cells are induced and accumulate in response to colonization with a subgroup of intestinal microbes such as segmented filamentous bacteria (SFB) and certain extracellular pathogens. Here, we show that adhesion of microbes to intestinal epithelial cells (ECs) is a critical cue for Th17 induction. Upon monocolonization of germ-free mice or rats with SFB indigenous to mice (M-SFB) or rats (R-SFB), M-SFB and R-SFB showed host-specific adhesion to small intestinal ECs, accompanied by host-specific induction of Th17 cells. Citrobacter rodentium and Escherichia coli O157 triggered similar Th17 responses, whereas adhesion-defective mutants of these microbes failed to do so. Moreover, a mixture of 20 bacterial strains, which were selected and isolated from fecal samples of a patient with ulcerative colitis on the basis of their ability to cause a robust induction of Th17 cells in the mouse colon, also exhibited EC-adhesive characteristics.

引用

页码：367 / 380

页数：14

共 36 条

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ATP drives lamina propria TH17 cell differentiation [J].