虫生真菌分子生物学研究进展

被引:10
作者
王成树
李增智
机构
[1] 安徽农业大学经济昆虫菌物研究所!合肥
关键词
虫生真菌; 蛋白酶; 基因克隆; 表达调控; 基因工程;
D O I
10.13344/j.microbiol.china.2001.03.021
中图分类号
Q933 [微生物遗传学];
学科分类号
071007 ;
摘要
虫生真菌分子生物学的研究自开展以来 ,有 2 0多种与杀虫毒力相关的蛋白酶得到纯化 ,而与之相对应的基因也分别得到克隆、测序。陆续的基因克隆表明 ,虫生具菌中的重要毒力基因以基因族的形式存在 ,在精确的表达调控下以适应不同的寄主种类和不同的环境条件。基因工程研究表明 ,以增加毒力基因拷贝的方式可显著提高虫生真菌的杀虫速率 ,带有外源基因标记的菌株也是环境释放示综研究的良好材料及方法。
引用
收藏
页码:88 / 92
页数:5
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