响应面法优化水芹黄酮提取工艺及其成分研究

被引:23
作者
陈况况
帕塔尔·尼牙孜
章宏慧
刘东红
叶兴乾
陈健初
机构
[1] 浙江大学生物系统工程与食品科学学院,馥莉食品研究院,浙江省农产品加工技术研究重点实验室,浙江省食品加工技术与装备工程中心
关键词
水芹; 黄酮; 响应面法; 高效液相色谱;
D O I
10.16429/j.1009-7848.2014.11.019
中图分类号
TS255.3 [果蔬加工与保藏];
学科分类号
083203 ;
摘要
采用水浴加热的方法研究水芹黄酮提取工艺。通过单因素试验分别考察料液比、提取溶剂浓度、加热温度和加热时间对水芹黄酮提取量的影响,确定各因素的适宜水平。在此基础上,利用Design-Expert软件中的Box-Behnken Design设计方法设计四因素三水平的试验方案,通过方差分析回归建立数学模型,确定水芹叶黄酮的最佳提取工艺条件是:料液比1∶25、乙醇体积分数70%、加热时间2.5 h、温度73.76℃。此条件下黄酮的提取量为31.478 mg/g(与预测值32.0041 mg/g接近)。使用相同条件从茎中提取的黄酮量为6.214 mg/g。采用高效液相色谱法分析水芹黄酮成分,结果水芹黄酮主要含芦丁、槲皮素-3-O-葡萄糖苷、槲皮素-3-L-鼠李糖苷、槲皮素、山奈酚。
引用
收藏
页码:83 / 89
页数:7
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