蛋白质突变体基因库构建方法的研究进展

[31] Growth factor engineering by degenerate homoduplex gene family recombination. Coco,W.M.,Encell,L.P.,Levinson,W.E.,Crist,M.J.,Loomis,A.K.,Licato,L.L.,Arensdorf,J.J.,Sica,N.,Pienkos,P.T.,Monticello,D.J. Nature Biotechnology . 2002

[32] Structure-based combinatorial protein engineering （SCOPE）. O’’’’Maille P E,Bakhtina M,TsaiM D. Journal of Molecular Biology . 2002

[33] Accurate and scalable identification of functional sites by evolutionary tracing. Lichtarge O, Yao H, Kristensen DM, et al. J Struct Funct Genomics . 2003

[34] Libraries of hybrid proteins from distantly related sequences. Sieber V, Martinez C A, Arnold F H. Nature Biotechnology . 2001

[35] Rapid generation of incremental truncation libraries for protein engineering using αphosphothioate nucleotides. Lutz S, Ostermeier M, Benkovic S J. Nucleic Acids Research . 2001

[36] Rapid evolution of beta-glucuronidase specificity by saturation mutagenesis of an active site loop. Geddie M,Matsumura I. Journal of Biological Chemistry . 2004

[37] Semi-rational approaches to engineering enzyme activity:combining the benefits of directed evolution and rational design. Chica, R. A,Doucet, N,Pelletier, J. N. Current Opinion in Biotechnology . 2005

[38] Directed evolution of enzyme catalysis. Kuchner O,Arnold F H. Trends in Biotechnology . 1997

[39] Random-priming in vitro recombination: an effective tool for directed evolution. Shao Z,Zhao H,Giver L,Arnold F H. Nucleic Acids Research . 1998

[40] Rapid revolution of a protein in vitro by DNA shuffling. Stemmer W P C. Nature . 1994

[31] Growth factor engineering by degenerate homoduplex gene family recombination. Coco,W.M.,Encell,L.P.,Levinson,W.E.,Crist,M.J.,Loomis,A.K.,Licato,L.L.,Arensdorf,J.J.,Sica,N.,Pienkos,P.T.,Monticello,D.J. Nature Biotechnology . 2002

[32] Structure-based combinatorial protein engineering （SCOPE）. O’’’’Maille P E,Bakhtina M,TsaiM D. Journal of Molecular Biology . 2002

[33] Accurate and scalable identification of functional sites by evolutionary tracing. Lichtarge O, Yao H, Kristensen DM, et al. J Struct Funct Genomics . 2003

[34] Libraries of hybrid proteins from distantly related sequences. Sieber V, Martinez C A, Arnold F H. Nature Biotechnology . 2001

[35] Rapid generation of incremental truncation libraries for protein engineering using αphosphothioate nucleotides. Lutz S, Ostermeier M, Benkovic S J. Nucleic Acids Research . 2001

[36] Rapid evolution of beta-glucuronidase specificity by saturation mutagenesis of an active site loop. Geddie M,Matsumura I. Journal of Biological Chemistry . 2004

[37] Semi-rational approaches to engineering enzyme activity:combining the benefits of directed evolution and rational design. Chica, R. A,Doucet, N,Pelletier, J. N. Current Opinion in Biotechnology . 2005

[38] Directed evolution of enzyme catalysis. Kuchner O,Arnold F H. Trends in Biotechnology . 1997

[39] Random-priming in vitro recombination: an effective tool for directed evolution. Shao Z,Zhao H,Giver L,Arnold F H. Nucleic Acids Research . 1998

[40] Rapid revolution of a protein in vitro by DNA shuffling. Stemmer W P C. Nature . 1994