饵料对蚯蚓粪细菌群落结构多样性的影响

被引：4

作者：

陈浩 ^{[1
,2
]}

赵海涛 ^{[1
]}

姚振飞 ^{[3
]}

孙红军 ^{[4
]}

胡健 ^{[1
]}

机构：

[1] 扬州大学环境科学与工程学院

[2] 江苏省农业科学院食品质量安全与检测研究所,农业部农产品质量安全控制技术与标准重点实验室

[3] 昆山市土壤肥料站

[4] 江苏东宝生物科技有限公司

来源：

农业环境科学学报 | 2012年 / 31卷 / 12期

关键词：

饵料; 蚯蚓粪; PCR-DGGE; 细菌群落多样性;

D O I：

暂无

中图分类号：

X172 [环境微生物学];

学科分类号：

摘要：

以牛粪、猪粪和污泥等有机废弃物作为蚯蚓饵料,利用细菌16SrDNAV3区特异扩增及变性梯度凝胶电泳(PCR-DGGE)技术,比较了不同饵料及饵料改变对蚯蚓粪细菌群落结构多样性的影响。结果表明:(1)与所取食的饵料相比,蚯蚓粪细菌群落结构多样性均有不同程度的下降,蚯蚓粪与对应饵料之间细菌群落结构相似性范围为15.3%～37.7%;(2)来自不同饵料的蚯蚓粪细菌群落结构相似性范围为17.2%～39.1%,其中以牛粪和猪粪为饵料时,蚯蚓粪细菌群落结构相似性最高,与对应的饵料细菌群落结构相似性相比,提高了近1倍;(3)当饵料由猪粪改为牛粪后,随着时间延长,蚯蚓粪细菌群落结构多样性逐渐下降并趋于稳定,并且蚯蚓粪与饵料牛粪的细菌群落结构相似性由0d的33.2%逐渐下降到21d的14.8%并趋于稳定。由此可知,饵料的种类及转换可以影响蚯蚓粪细菌群落结构多样性。

引用

页码：2500 / 2505

页数：6

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