湘南红壤稻田AOA-amoA、narG、nosZ基因丰度及其环境影响因子

被引：8

作者：

高嵩涓 ^{[1
]}

曹卫东 ^{[1
,2
]}

白金顺 ^{[1
]}

高菊生 ^{[1
,3
]}

黄晶 ^{[1
,3
]}

曾闹华 ^{[1
]}

常单娜 ^{[1
]}

志水胜好 ^{[4
]}

机构：

[1] 中国农业科学院农业资源与农业区划研究所/农业部植物营养与肥料重点实验室

[2] 青海大学青海省农林科学院土壤肥料研究所

[3] 中国农业科学院衡阳红壤实验站

[4] 鹿儿岛大学农学部

来源：

中国土壤与肥料 | 2017年 / 01期

关键词：

红壤稻田; 硝化作用; 反硝化作用; 环境因子; 功能基因;

D O I：

暂无

中图分类号：

S154 [土壤生物学];

学科分类号：

071012 ; 0713 ;

摘要：

为了解南方红壤稻田参与土壤氮循环过程的功能微生物及其与环境因子间的普遍规律性关系,应用荧光定量PCR方法研究了稻田中不同时期氨氧化古菌氨单加氧酶编码基因AOA-amoA、硝酸盐还原酶编码基因narG和N2O还原酶编码基因nosZ基因丰度的变化,结合主成分分析（PCA）、冗余分析（RDA）探讨了有关硝化、反硝化微生物功能基因丰度变化及其与土壤环境因子间的相互关系。结果表明:AOA-amoA、narG和nosZ基因丰度间极显著正相关;AOA-amo A、narG和nosZ基因丰度随水稻生育进程逐渐降低,在早稻成熟期显著低于翻地前及早稻移栽前。土壤有机质、电导率、微生物生物量碳、微生物生物量氮、pH值、Eh及盐酸提取态Fe（Ⅱ）对AOA-amoA、narG和nosZ基因丰度的变化有重要影响,其中盐酸提取态Fe（Ⅱ）与其相关性最强。本研究探讨了红壤稻田氮转化微生物在不同时期的数量变化及其环境影响因素,为合理调控红壤稻田氮转化提供了理论依据。

引用

页码：21 / 27

页数：7

共 22 条

[11]

Ammonia‐oxidizing archaea: important players in paddy rhizosphere soil?[J] . Xue‐PingChen,YueXia,Ju‐PeiShen.Environmental Microbiology . 2008 (8)

[12]

Relationship between nitrifier and denitrifier community composition and abundance in predicting nitrous oxide emissions from ephemeral wetland soils[J] . W.K. Ma,A. Bedard-Haughn,S.D. Siciliano,R.E. Farrell.Soil Biology and Biochemistry . 2007 (5)

[13]

Soil enzyme activities, microbial community composition and function after 47 years of continuous green manuring[J] . Applied Soil Ecology . 2006 (3)

[14]

Effects of management regime and plant species on the enzyme activity and genetic structure of N‐fixing, denitrifying and nitrifying bacterial communities in grassland soils[J] . Ashok K.Patra,LucAbbadie,AnnieClays‐Josserand,ValérieDegrange,Susan J.Grayston,NadineGuillaumaud,PierreLoiseau,FrédériqueLouault,ShahidMahmood,SylvieNazaret,LaurentPhilippot,FranckPoly,James I.Prosser,Xavier LeRoux.Environmental Microbiology . 2006 (6)

[15]

Nitrification–denitrification dynamics and community structure of ammonia oxidizing bacteria in a high yield irrigated Philippine rice field[J] . Mette Haubjerg Nicolaisen,Nils Risgaard-Petersen,Niels Peter Revsbech,Wolfgang Reichardt,Niels Birger Ramsing.FEMS Microbiology Ecology . 2004 (3)

[16] Denitrifying genes in bacterial and Archaeal genomes [J].

Philippot, L .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2002, 1577 (03) :355-376

[17]

AMMONIA-OXIDIZING BACTERIA: A Model for Molecular Microbial Ecology[J] . George A. Kowalchuk,John R. Stephen.Annual Review of Microbiology . 2001 (1)

[18] Nitrate-dependent iron(II) oxidation in paddy soil [J].

Ratering, S ;

Schnell, S .

ENVIRONMENTAL MICROBIOLOGY, 2001, 3 (02) :100-109

[19]

Nitrification and denitrification in the rhizosphere of rice: the detection of processes by a new multi-channel electrode[J] . I. Arth,P. Frenzel.Biology and Fertility of Soils . 2000 (5)

[20] Molecular analysis of ammonia oxidation and denitrification in natural environments [J].

Bothe, H ;

Jost, G ;

Schloter, M ;

Ward, BB ;

Witzel, KP .

FEMS MICROBIOLOGY REVIEWS, 2000, 24 (05) :673-690

← 1 2 3 →