变性梯度凝胶电泳在环境微生物生态学中的应用

被引:10
作者
单国彬 [1 ]
金文标 [2 ]
林佶侃 [1 ]
邢新会 [3 ]
机构
[1] 东莞宝丽美化工有限公司
[2] 哈尔滨工业大学深圳研究生院城市与土木工程学院
[3] 清华大学化学工程系生物化工研究所
关键词
PCR-变性梯度凝胶电泳(PCR-DGGE); 废水生物处理; 微生物群落; 活性污泥; 生物膜; 硝化菌-反硝化菌; 硫酸还原菌;
D O I
10.13292/j.1000-4890.2006.0239
中图分类号
Q938-3 [];
学科分类号
071005 ; 100705 ;
摘要
PCR-变性梯度凝胶电泳(PCR-DGGE)具有可靠性强、重复性好、方便快捷等优点,已被广泛应用于环境生态学中微生物群落多样性、动态性分析和功能细菌的跟踪。本文综述了PCR-DGGE技术的基本原理,不同DNA提取方法的比较,不同PCR方式的比较及其在环境生态学中研究微生物群落多样性、环境中微生物群落变化的动态监测、硝化菌-反硝化菌和硫酸还原菌(SRB)的动态分析和监测等领域中的应用,并对该技术自身存在的局限性和应用前景进行了评价。
引用
收藏
页码:1257 / 1264
页数:8
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