三磷腺苷合酶抑制因子1基因敲除对小鼠成纤维细胞中三磷腺苷水平及脂肪细胞分化的影响

被引：4

作者：

关丫丫 ^{[1
,2
]}

梁银明 ^{[1
,2
]}

王辉 ^{[1
,2
]}

叶建平 ^{[1
,2
,3
]}

机构：

[1] 新乡医学院医学检验学院

[2] 河南省分子诊断与医学检验技术协同创新中心

[3] 美国路易斯安那州立大学Pennington生物医学中心

来源：

新乡医学院学报 | 2018年 / 35卷 / 08期

关键词：

ATP合酶抑制因子1; 脂肪细胞分化; 三磷腺苷; 三酰甘油;

D O I：

暂无

中图分类号：

R329.2 [人体细胞学];

学科分类号：

摘要：

目的探讨三磷腺苷合酶抑制因子1(ATPIF1)基因敲除对小鼠成纤维细胞中三磷腺苷(ATP)水平及脂肪细胞分化的影响。方法取5只ATPIF1基因敲除小鼠作为观察组,另取5只C57BL/6小鼠作为对照组。取各组小鼠耳组织,采用Ⅰ型胶原酶和中性蛋白酶Ⅱ的消化作用制备成纤维细胞并用高糖培养基培养,传代1～2次后,待细胞长满且接触抑制3 d后,更换含白色脂肪细胞诱导剂Ⅰ的细胞培养基诱导4 d,再更换含白色脂肪细胞诱导剂Ⅱ的细胞培养基诱导4 d。在诱导前及诱导4、8 d分别采用ATP检测试剂盒和三酰甘油(TG)检测试剂盒检测成纤维细胞中ATP和TG水平。结果诱导前,对照组小鼠原代成纤维细胞中ATP水平明显低于观察组(P<0.05);诱导4、8 d,对照组与观察组小鼠原代成纤维细胞中ATP水平比较差异无统计学意义(P>0.05)。诱导4、8 d,对照组小鼠原代成纤维细胞中TG水平低于观察组(P<0.05)。结论 ATPIF1基因敲除可增加小鼠成纤维细胞中ATP水平,促进成纤维细胞向白色脂肪细胞分化,提高成熟白色脂肪细胞储存TG的能力。

引用

页码：658 / 661

页数：4

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