长期施肥制度对稻田土壤反硝化细菌群落活性和结构的影响

被引：27

作者：

陈哲 ^{[1
,2
]}

袁红朝 ^{[1
,2
]}

吴金水 ^{[1
]}

魏文学 ^{[1
]}

机构：

[1] 中国科学院亚热带农业生态研究所

[2] 中国科学院研究生院

来源：

生态学报 | 2009年 / 29卷 / 11期

关键词：

水稻土; 施肥; 反硝化速率; narG基因; 多样性;

D O I：

暂无

中图分类号：

S154.3 [土壤微生物学];

学科分类号：

摘要：

以中国科学院桃源农业生态试验站长期定位施肥试验为平台,研究了3种长期施肥制度(对照不施肥-CK,化学施肥-NPK,化学施肥+有机肥-NPKOM)下土壤反硝化速率的差异。同时,以硝酸还原酶基因(narG)作为反硝化细菌的功能标志物,分析了施肥对反硝化细菌群落结构和多样性的影响。结果表明,长期施用有机肥的土壤反硝化速率,反硝化菌多样性都高于对照和施用化肥处理。从3个处理的土壤样品中共获得35个narG基因的可操作分类单元(OTU)主要分布在两个簇,与变形菌门(Proteobacteria)和放线菌门(Actinobacteria)的反硝化细菌有一定的亲缘关系,均为首次从土壤中克隆。Shannon多样性指数显示,NPKOM处理的narG基因多样性最高,CK处理次之,NPK处理最低。LUBSHUFF软件对narG基因群落组成的分析显示,施有机肥后含narG基因的细菌群落组成与CK之间有显著性差异(P<0.05),而化肥(NPK)没有产生显著影响。实验结果为进一步研究亚热带地区水稻土反硝化作用及反硝化功能菌提供了重要的依据。

引用

页码：5923 / 5929

页数：7

共 10 条

[1] 红树林土壤总DNA不同提取方法比较研究 [J].

杨建 ;

洪葵 .

生物技术通报, 2006, (S1) :366-371

[2] 农田土壤N2O排放研究进展 [J].

黄树辉 ;

吕军 .

土壤通报, 2004, (04) :516-522

[3]

Bacterial diversity of soils assessed by DGGE, T-RFLP and SSCP fingerprints of PCR-amplified 16S rRNA gene fragments: Do the different methods provide similar results?[J] . Kornelia Smalla,Miruna Oros-Sichler,Annett Milling,Holger Heuer,Susanne Baumgarte,Regina Becker,Gabriele Neuber,Siegfried Kropf,Andreas Ulrich,Christoph C. Tebbe.Journal of Microbiological Methods . 2007 (3)

[4]

Community composition and activities of denitrifying bacteria from adjacent agricultural soil, riparian soil, and creek sediment in Oregon, USA[J] . Jeremy J Rich,David D Myrold.Soil Biology and Biochemistry . 2004 (9)

[5] Observation of high seasonal variation in community structure of denitrifying bacteria in arable soil receiving artificial fertilizer and cattle manure by determining T-RFLP of nir gene fragments [J].

Wolsing, M ;

Priemé, A .

FEMS MICROBIOLOGY ECOLOGY, 2004, 48 (02) :261-271

[6] Genetic characterization of the nitrate reducing community based on narG nucleotide sequence analysis [J].

Chèneby, D ;

Hallet, S ;

Mondon, A ;

Martin-Laurent, F ;

Germon, JC ;

Philippot, L .

MICROBIAL ECOLOGY, 2003, 46 (01) :113-121

[7]

Temporal variation in N 2 O and N 2 fluxes from a permanent pasture in Switzerland in relation to management, soil water content and soil temperature[J] . Agriculture, Ecosystems and Environment . 1999 (1)

[8]

Nitrous oxide release from arable soil: Importance of N-fertilization, crops and temporal variation[J] . E.-A. Kaiser,K. Kohrs,M. Kücke,E. Schnug,O. Heinemeyer,J.C. Munch.Soil Biology and Biochemistry . 1998 (12)

[9] Potential denitrification activity assay in soil - With or without chloramphenicol? [J].

Pell, M ;

Stenberg, B ;

Stenstrom, J ;

Torstensson, L .

SOIL BIOLOGY & BIOCHEMISTRY, 1996, 28 (03) :393-398

[10] DENITRIFICATION IN THE TOP-SOIL OF MANAGED GRASSLANDS IN THE NETHERLANDS IN RELATION TO SOIL TYPE AND FERTILIZER LEVEL [J].

DEKLEIN, CAM ;

VANLOGTESTIJN, RSP .

PLANT AND SOIL, 1994, 163 (01) :33-44

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