雷公藤甲素对MIA模型大鼠膝骨关节炎c-Jun、MMP-9表达及血清炎性标志物的影响

被引：12

作者：

陈晓昱

机构：

[1] 沈阳市骨科医院药剂科

来源：

实用药物与临床 | 2015年 / 18卷 / 11期

关键词：

骨关节炎; c-Jun; MMP-9; PGE2; IL-8; 雷公藤甲素;

D O I：

10.14053/j.cnki.ppcr.201511006

中图分类号：

R285.5 [中药实验药理]; R-332 [医用实验动物学];

学科分类号：

100402 [劳动卫生与环境卫生学]; 100806 [中药药理学];

摘要：

目的观察雷公藤甲素对膝部骨关节炎滑膜c-Jun、MMP-9及血清PGE2、IL-8水平的影响,探讨其在膝部骨关节炎中的药理学价值。方法 45只SD大鼠随机分为正常对照组、模型对照组及雷公藤甲素组,建立碘乙酸钠(Monosodium iodoacetate,MIA)膝关节炎模型,并给予雷公藤甲素干预,以Western blot及(qRT)-PCR法检测c-Jun及M M P-9在膝关节滑膜中蛋白及mRNA水平表达,以酶联免疫法检测外周血中PGE2及IL-8水平。结果 Western blot及(qRT)-PCR显示,c-Jun及MMP-9在膝关节滑膜组织中蛋白及mRNA水平上均有表达,雷公藤甲素组及正常对照组明显低于模型对照组(P<0.01),雷公藤甲素组与正常对照组比较差异无统计学意义(P>0.05);在血清PGE2及IL-8表达上,雷公藤甲素组及正常对照组明显低于模型对照组(P<0.05)。结论雷公藤甲素可抑制骨关节炎滑膜中c-Jun及MMP-9表达,降低外周血PGE2及IL-8水平,抑制炎症反应,对骨关节炎有治疗作用。

引用

页码：1293 / 1296

页数：4

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