高效液相色谱法测定3种植物油中4种黄曲霉毒素含量

被引：11

作者：

祝伟霞 ^{[1
]}

杨冀州 ^{[1
]}

袁萍 ^{[1
]}

王彩娟 ^{[2
]}

魏蔚 ^{[1
]}

机构：

[1] 河南出入境检验检疫局

[2] 郑州大学化学系

来源：

理化检验(化学分册) | 2013年 / 49卷 / 08期

关键词：

黄曲霉毒素; 高效液相色谱荧光检测法; 植物油;

D O I：

暂无

中图分类号：

TS227 [产品标准与检验]; O657.72 [];

学科分类号：

083202 ; 070302 ; 081704 ;

摘要：

提出了高效液相色谱法测定花生油、橄榄油和芝麻油等3种植物油中4种黄曲霉毒素B1、B2、G1和G2的含量。样品采用55%（体积分数）甲醇溶液提取后,经免疫亲和柱净化,流出液中的黄曲霉毒素在YMC ODS-AQ S-3色谱柱上用含溴化钾120mg和硝酸350μL的甲醇-乙腈-水（25+17+60）混合液作流动相进行色谱分离,并经衍生化后进行荧光检测,所用激发及发射波长依次为362,440nm。4种黄曲霉素的质量浓度分别在一定范围内与其对应的峰面积呈线性关系,黄曲霉毒素B1、G1、B2和G2的测定下限（10S/N）依次为0.4,0.4,0.2,0.2μg·kg-1。在3个浓度水平下进行加标回收试验,方法的回收率在74%112%之间,测定值的相对标准偏差（n=10）在3.6%15%之间。

引用

页码：981 / 984

页数：4

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