重离子辐照诱变阿佛曼链霉菌的实验观察

被引：2

作者：

尚勇良 ^{[1
,2
]}

杨进才 ^{[1
]}

卫广森 ^{[1
]}

贾宁 ^{[2
]}

机构：

[1] 中农威特生物科技股份有限公司

[2] 甘肃农业大学

来源：

现代生物医学进展 | 2009年 / 9卷 / 15期

关键词：

阿维菌素; 阿佛曼链霉菌; 诱变手段; 重离子束辐照;

D O I：

10.13241/j.cnki.pmb.2009.15.012

中图分类号：

Q937 [微生物生物物理学];

学科分类号：

071011 ;

摘要：

目的:对阿佛曼链霉菌采用新的诱变手段,以获得稳定高产的优良菌株。方法:采用重离子束辐照阿佛曼链霉菌,研究了0.25Gy、0.5Gy、3Gy、5Gy、10Gy和15Gy剂量的12C+粒子束辐照阿佛曼链霉菌菌株后,菌落特性的变化及对菌株产素能力的影响。结果:重离子辐照阿佛曼链霉菌后,在其各个辐照剂量区都存在变异菌株,诱变后阿佛曼链霉菌的菌落形态多样,小山状,火山口状、彗星尾状、车轮状、边缘放射状等;菌落大小不一,有的直径达4～5mm,有的小如针尖状。效价提高到7298μg/mL,获得了高产菌株。结论:重离子束辐照阿佛曼链霉菌菌株后,阿佛曼链霉菌的产素能力显著提高,可得到高产的菌株。

引用

页码：2844 / 2846

页数：3

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