数字PCR技术的发展和应用

被引：38

作者：

詹成 ^{[1
]}

燕丽 ^{[2
]}

王琳 ^{[1
]}

金玉麟 ^{[1
]}

陈力 ^{[1
]}

时雨 ^{[1
]}

王群 ^{[1
]}

机构：

[1] 复旦大学附属中山医院胸外科

[2] 复旦大学附属眼耳鼻喉科医院放疗科

来源：

复旦学报(医学版) | 2015年 / 42卷 / 06期

基金：

上海市自然科学基金;

关键词：

数字PCR; 微滴式dPCR; 芯片式dPCR; 突变检测; 拷贝数变异; 基因表达;

D O I：

暂无

中图分类号：

Q503 [生物化学技术];

学科分类号：

摘要：

数字PCR(digital PCR,dPCR)技术作为一种全新的核酸检测方法,通过把反应体系均分到大量反应单元中独立地进行PCR,并根据泊松分布和阳性比例来计算核酸数量。目前dPCR主要分为微滴式和芯片式两种。与传统PCR技术相比,dPCR具有高灵敏度、高精确度、高耐受性和绝对定量的优点。因此,dPCR技术在近年来得到了迅速的发展,广泛地应用于稀有突变检测、拷贝数变异分析和复杂样本基因表达检测等方面。

引用

页码：786 / 789

页数：4

共 14 条

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