高效液相色谱法测定紫甘薯花青素含量

被引：56

作者：

毛建霏

周虹

雷绍荣

郭灵安

欧阳华学

代晓航

机构：

[1] 四川省农业科学院分析测试中心农业部食品质量监督检验测试中心

来源：

西南农业学报 | 2012年 / 25卷 / 01期

关键词：

高效液相色谱; 紫甘薯; 花色苷; 花青素; 矢车菊; 芍药素; 提取; 水解;

D O I：

暂无

中图分类号：

S531 [甘薯（红薯）];

学科分类号：

090104 [作物信息科学与技术];

摘要：

本文建立了紫甘薯中花青素的高效液相色谱-紫外定量检测方法。使用盐酸水溶液超声提取紫甘薯花色苷,沸水浴将多种花色苷水解为花青素单体。采用高效液相色谱-紫外可见检测器对紫甘薯中主要花青素矢车菊和芍药素进行定量检测。结果表明:矢车菊素线性范围0.0454～90.8μg/mL,仪器定量限26.7 ng/mL,仪器检出限8.0 ng/mL,方法检出限0.80 mg/kg,日内RSD 1.8%,回收率97.8%～100.7%;芍药素线性范围0.0420～80.4μg/mL,仪器定量限24.7 ng/mL,仪器检出限7.4 ng/mL,方法检出限0.74 mg/kg,日内RSD 2.4%回收率93.7%～95.5%。该方法便捷准确,可将紫甘薯及其他作物中复杂花色苷水解为花青素单体进行定量测定。

引用

页码：123 / 127

页数：5

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