氮添加对杨树人工林表层土壤微生物群落结构的影响

被引:17
作者
赵超 [1 ]
王文娟 [1 ]
阮宏华 [1 ]
葛之葳 [1 ]
徐长柏 [2 ]
曹国华 [2 ]
机构
[1] 南京林业大学
[2] 江苏省东台市国营林场
关键词
土壤微生物; 氮添加; 微生物群落结构; 磷脂脂肪酸法; 杨树人工林;
D O I
10.13759/j.cnki.dlxb.20150522.051
中图分类号
S792.11 [杨]; S714.3 [森林土壤生物学];
学科分类号
090708 [林木遗传育种学]; 090709 [森林培育学];
摘要
以江苏东台沿海地区杨树人工林地为研究对象,建立野外模拟氮沉降试验样地,试验设置5个氮处理水平,分别为对照(0 kg·hm-2·a-1)、低氮(50 kg·hm-2·a-1)、中氮(100 kg·hm-2·a-1)、高氮(150 kg·hm-2·a-1)和超高氮(300 kg·hm-2·a-1)。于2012年5月开始在生长季(5—10月)每月进行施氮处理,2013年8月和10月运用磷脂脂肪酸法分别分析了土壤微生物群落的变化特征。结果表明,施氮处理近2 a后,两个月份中的土壤微生物磷脂脂肪酸总量均显著下降,细菌、真菌中磷脂脂肪酸的量出现了不同程度的下降,真菌与细菌中磷脂脂肪酸的量之比没有显著变化。磷脂脂肪酸组成的主成分分析显示,除低氮处理样地外,其他水平施氮样地均使微生物群落结构显著变化,且超高氮处理样地变化格局具有季节差异性。
引用
收藏
页码:83 / 88
页数:6
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