实时荧光定量PCR的发展和数据分析

[31] Detection and monitoring of virusinfections by real-time PCR. Watzinger F,Ebner K,Lion T. Molecular Aspects of Medicine . 2006

[32] Quantitative RT-PCR: pitfalls and potential. Freeman W M, Walker S J, Vrana K E. Biotechniques . 1999

[33] Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. Schmittgen TD, Zakrajsek BA, Mills AG, et al. Analytical Biochemistry . 2000

[34] Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences. Bustin SA. Expert Review of Molecular Diagnostics . 2005

[35] Analyzing real-time PCR data by the comparative C（T）method. Schmittgen T D,Livak K J. Nat Protoc . 2008

[36] Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. Schmittgen TD, Zakrajsek BA. Journal of Biochemistry . 2000

[37] Real-time PCR assay for detection and quantification of hepatitis B virus genotypes A to G. Welzel TM,Miley WJ,Parks TL,Goedert JJ,Whitby D,Ortiz- Conde BA. Journal of Clinical Microbiology . 2006

[38] Real-time PCRquantitation of hepatitis B virus total DNA and covalently closed circularDNA in peripheral blood mononuclear cells from hepatitis Bvirus-infected patients. Mazet-Wagner AA,Baclet MC,Loustaud-Ratti V,et al. Journal of Virological Methods . 2006

[39] RNA isolation and real-time quantitative RT-PCR. Guan H,Yang K. Methods in Molecular Biology . 2008

[31] Detection and monitoring of virusinfections by real-time PCR. Watzinger F,Ebner K,Lion T. Molecular Aspects of Medicine . 2006

[32] Quantitative RT-PCR: pitfalls and potential. Freeman W M, Walker S J, Vrana K E. Biotechniques . 1999

[33] Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. Schmittgen TD, Zakrajsek BA, Mills AG, et al. Analytical Biochemistry . 2000

[34] Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences. Bustin SA. Expert Review of Molecular Diagnostics . 2005

[35] Analyzing real-time PCR data by the comparative C（T）method. Schmittgen T D,Livak K J. Nat Protoc . 2008

[36] Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. Schmittgen TD, Zakrajsek BA. Journal of Biochemistry . 2000

[37] Real-time PCR assay for detection and quantification of hepatitis B virus genotypes A to G. Welzel TM,Miley WJ,Parks TL,Goedert JJ,Whitby D,Ortiz- Conde BA. Journal of Clinical Microbiology . 2006

[38] Real-time PCRquantitation of hepatitis B virus total DNA and covalently closed circularDNA in peripheral blood mononuclear cells from hepatitis Bvirus-infected patients. Mazet-Wagner AA,Baclet MC,Loustaud-Ratti V,et al. Journal of Virological Methods . 2006

[39] RNA isolation and real-time quantitative RT-PCR. Guan H,Yang K. Methods in Molecular Biology . 2008