儿童芒果果实过敏原研究

被引：1

作者：

曹会 ^{[1
]}

蔡德丰 ^{[2
]}

许晓璇 ^{[1
]}

杨平常 ^{[1
]}

刘志刚 ^{[1
]}

夏立新 ^{[1
]}

机构：

[1] 深圳大学医学院呼吸疾病国家重点实验室深圳大学变态反应分室

[2] 深圳市儿童医院检验科

来源：

南昌大学学报(医学版) | 2017年 / 57卷 / 04期

关键词：

儿童; 芒果; 过敏原图谱; 双向电泳; 免疫印迹;

D O I：

10.13764/j.cnki.ncdm.2017.04.004

中图分类号：

R446.6 [免疫学检验]; R725.9 [小儿全身性疾病];

学科分类号：

100208 ; 100202 ;

摘要：

目的探讨儿童芒果过敏患者的芒果果实过敏原。方法提取芒果果实蛋白质组分,利用十二烷基丙磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)对粗提蛋白进行组分分析;双向电泳-免疫印迹法鉴定芒果过敏原成分。结果芒果果实中100ku及35ku蛋白与儿童芒果过敏患者血清IgE反应率较高(6/14),双向电泳-免疫印迹结果显示与IgE反应较为明显的有至少12个蛋白点,这些蛋白等电点均大于6.5。结论儿童芒果过敏原与成人有差异。

引用

页码：14 / 16+20 +20

页数：4

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