基于钴酞菁聚合物膜修饰电极的生物传感器测定有机磷农药的研究

被引：3

作者：

康天放

高慧丽

机构：

[1] 北京工业大学环境与能源工程学院,北京工业大学环境与能源工程学院北京,北京

来源：

化学传感器 | 2005年 / 01期

关键词：

生物传感器; 乙酰胆碱酯酶; 四氨基钴酞菁; 有机磷农药;

D O I：

暂无

中图分类号：

TP212.3 [生物传感器、医学传感器];

学科分类号：

080202 ;

摘要：

该文报道了一种用于检测有机磷农药的安培生物传感器,将钴（Ⅱ）-4,4',4'',4'''四氨基酞菁（CoTAPc）单体电聚合到一支玻碳电极上作为硫代胆碱电化学氧化的电催化剂,通过交联的方法将乙酰胆碱酯酶（AChE）固定在醋酸纤维膜上,再将该酶膜固定于聚四氨基钴酞菁（p-CoTAPc）修饰电极表面。采用10min 的温育时间,百分抑制率与对硫磷或乐果浓度的对数值都在1.0×10-9～1.0×10-6mol/L 浓度范围内呈线性关系,检测限分别可达7.0×10-10mol/L 和2.6×10-10mol/L。农药对 AChE 的抑制作用产生后,用2-吡啶醛肟甲基氯化物（2-PAM）可以有效地使酶膜再生活化。

引用

页码：52 / 56

页数：5

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