SOX2基因对骨关节炎软骨细胞凋亡的影响研究

被引：12

作者：

张玉昌 ^{[1
]}

赵萍 ^{[1
]}

慕向前 ^{[1
]}

蒋宜伟 ^{[2
]}

机构：

[1] 甘肃省中医院/甘肃中医药大学第一附属医院骨科

[2] 甘肃中医药大学中医临床学院中医骨伤基础教研室

来源：

中国骨质疏松杂志 | 2019年 / 25卷 / 12期

关键词：

骨关节炎; 软骨细胞; SOX2基因; 凋亡; JAK2/STAT3信号通路;

D O I：

暂无

中图分类号：

R684.3 [关节炎];

学科分类号：

100220 [骨科学];

摘要：

目的探讨SOX2基因对人骨关节炎（OA）软骨细胞凋亡的影响及机制。方法以正常软骨组织作为对照,通过Western blotting检测OA软骨组织SOX2蛋白表达。从人OA中分离软骨细胞,参照LipofectamineTM2000说明将重组体pcDNA3.1-SOX2及空载体pcDNA3.1转染软骨细胞,并设置空白对照组。AG490作为JAK2/STAT3信号通路抑制剂,各组细胞处理48 h,通过流式细胞术、ROS试剂盒分别检测各组细胞凋亡率及ROS水平。Western blotting检测JAK2、p-JAK2、STAT3和p-STAT3的蛋白相对表达量。结果人OA软骨组织SOX2表达明显低于在正常软骨组织表达（0.065±0.009 vs 0.313±0.028,P<0.05）。转染pcDNA3.1-SOX2的OA软骨细胞SOX2表达明显高于空白组（0.556±0.048 vs 0.122±0.013,P<0.05）。pcDNA3.1-SOX2可明显降低OA软骨细胞凋亡率（3.11±0.42 vs 8.54±0.68）及ROS水平（23.46±2.15 vs 52.67±4.41）,上调p-JAK2（0.142±0.013 vs 0.065±0.009）和p-STAT3表达（0.218±0.020 vs 0.126±0.015）(P<0.05),AG490（15.23±1.13 vs 8.15±0.62）可诱导OA软骨细胞凋亡,而pcDNA3.1-SOX2可减弱AG490对OA软骨细胞凋亡促进作用（P<0.05）。结论 SOX2可抑制OA软骨细胞凋亡,其机制可能与激活JAK2/STAT3信号通路有关。

引用

页码：1773 / 1777

页数：5

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