紫草素对白介素-22介导永生化角质形成细胞株增殖、迁移及分化的影响

被引：5

作者：

朱晓芳 ^{[1
]}

林向飞 ^{[1
]}

刘国华 ^{[1
]}

陈菲菲 ^{[1
]}

刘燕茹 ^{[1
]}

孟松树 ^{[2
]}

卜平 ^{[3
]}

机构：

[1] 扬州大学临床医学院皮肤科

[2] 扬州大学生命科学与技术学院

[3] 扬州大学医学院中西医结合临床系

来源：

临床皮肤科杂志 | 2014年 / 43卷 / 04期

关键词：

紫草素; IL-22; 角质形成细胞;

D O I：

10.16761/j.cnki.1000-4963.2014.04.015

中图分类号：

R758.63 [牛皮癣（银屑病）];

学科分类号：

100206 ;

摘要：

目的:研究紫草素对白介素(IL)-22刺激永生化角质形成细胞株HaCaT细胞增殖、迁移及分化的影响。方法:WST-1法检测紫草素对II-22刺激HaCaT细胞增殖的作用;Transwell法检测紫草素对IL-22刺激HaCaT细胞迁移的影响;Western blot法检测紫草素对IL-22刺激后HaCaT细胞分化相关蛋白兜甲蛋白(loricrin)表达的影响。结果:紫草素可抑制IL-22引起的HaCaT细胞增殖和移动;促进了IL-22刺激后HaCaT细胞分化相关蛋白的表达。结论:紫草素对IL-22介导的体外培养HaCaT细胞增殖及迁移有抑制作用并促进细胞分化。

引用

页码：215 / 218

页数：4

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