枸杞nrDNA ITS测序鉴定的初步研究（英文）

被引：9

作者：

石志刚

安巍

樊云芳

焦恩宁

赵建华

王亚军

机构：

[1] 宁夏枸杞工程技术研究中心

来源：

AgriculturalScience&Technology | 2008年 / 01期

关键词：

Lycium L; ITS sequence; DNA sequencing; Identification;

D O I：

10.16175/j.cnki.1009-4229.2008.01.011

中图分类号：

S567.19 [];

学科分类号：

1008 ;

摘要：

[Objective] The study aimed to identify wolfberry(Lycium Linn.)germplasm resources at molecular level by analyzing the nrDNA ITS sequence.[Method] Genomic DNA from wolfberry leaves extracted by modified CTAB method were regarded as templates for PCR amplification by specific primer,clone and sequencing.[Result] The nrDNA ITS sequences were obtained and then differentiated among three tested materials.[Conclusion] PCR amplification and sequencing on nrDNA ITS is a feasible approach to identify different wolfberry germplasm resources.

引用

页码：35 / 38

页数：4

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[3]

Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues[J] . Scott O. Rogers,Arnold J. Bendich.Plant Molecular Biology . 1985 (2)

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