远程缺血后适应对脑缺血再灌注大鼠血脑屏障的保护作用

被引：7

作者：

杨世亮 ^{[1
]}

周晓明 ^{[2
]}

谭帅 ^{[1
]}

黄欣怡 ^{[1
]}

洪华 ^{[1
]}

机构：

[1] 中山大学附属第一医院神经科

[2] 中山大学附属第六医院康复科

来源：

中山大学学报(医学科学版) | 2015年 / 36卷 / 03期

基金：

广东省自然科学基金;

关键词：

脑缺血再灌注; 远程缺血后适应; 血脑屏障; 紧密连接;

D O I：

暂无

中图分类号：

R743 [脑血管疾病];

学科分类号：

100204 [神经病学];

摘要：

【目的】研究在大鼠脑缺血再灌注(IR)损伤模型中远程缺血后适应对血脑屏障的作用及相关机制。【方法】将SD大鼠96只随机分为3组:假手术组、单纯缺血再灌注组和远程缺血后适应组。利用线栓法制作大鼠脑IR模型,缺血1.5 h后恢复再通,再灌注72 h后对各组大鼠进行神经功能评分,染色检测脑梗死体积、梗死侧大脑半球脑组织伊文思蓝(EB)渗出量,再对紧密连接相关蛋白Claudin-5和Occludin的表达水平进行分析。【结果】与假手术组相比,单纯缺血再灌注组神经功能评分(9.9±2.0)、脑梗死体积(58.8±1.4)及EB的渗出量(5.7±0.6)均增加(P<0.01),同时Claudin-5和Occludin表达下降(P<0.01);与单纯缺血再灌注组比较,远程缺血后适应组大鼠神经功能评分(7.5±1.7)降低(P<0.01),脑梗死体积(33±5)减少(P<0.01),EB渗出量(2.7±1.2)减少(P<0.01),相应地Claudin-5和Occludin表达增加(P<0.01)。【结论】远程缺血后适应可以减轻脑缺血再灌注损伤,维持血脑屏障稳定性,可能与增加Claudin-5、Occludin的表达有关。

引用

页码：371 / 376

页数：6

共 12 条

[1]

The beneficial effects of postconditioning on no-reflow phenomenon after percutaneous coronary intervention in patients with ST-elevation acute myocardial infarction [J].

Dong, Mei ;

Mu, Nan ;

Guo, Fangming ;

Zhang, Chuanhuan ;

Ren, Faxin ;

Li, Jianping ;

Tao, Zhigang ;

Yang, Jun ;

Li, Guangping .

JOURNAL OF THROMBOSIS AND THROMBOLYSIS, 2014, 38 (02) :208-214

[2]

Neuroprotective mechanism of ischemic postconditioning in mice: a possible relationship between protein kinase C and nitric oxide pathways [J].

Gulati, Puja ;

Singh, Nirmal .

JOURNAL OF SURGICAL RESEARCH, 2014, 189 (01) :174-183

[3]

The science and clinical translation of remote postconditioning [J].

Vinten-Johansen, Jakob ;

Shi, Weiwei .

JOURNAL OF CARDIOVASCULAR MEDICINE, 2013, 14 (03) :206-213

[4]

Normobaric hyperoxia combined with minocycline provides greater neuroprotection than either alone in transient focal cerebral ischemia.[J].Xinchun Jin;Jie Liu;Ke J. Liu;Gary A. Rosenberg;Yi Yang;Wenlan Liu.Experimental Neurology.2013,

[5]

Protective Effect of Luteolin in Experimental Ischemic Stroke: Upregulated SOD1, CAT, Bcl-2 and Claudin-5, Down-Regulated MDA and Bax Expression [J].

Qiao, Huimin ;

Dong, Lipeng ;

Zhang, Xiangjian ;

Zhu, Chunhua ;

Zhang, Xiaolin ;

Wang, Lina ;

Liu, Zongjie ;

Chen, Linyu ;

Xing, Yinxue ;

Wang, Chaohui ;

Li, Yanhua .

NEUROCHEMICAL RESEARCH, 2012, 37 (09) :2014-2024

[6]

Post-Conditioning Reduces Infarct Size and Edema in Patients With ST-Segment Elevation Myocardial Infarction [J].

Thuny, Franck ;

Lairez, Olivier ;

Roubille, Francois ;

Mewton, Nathan ;

Rioufol, Gilles ;

Sportouch, Catherine ;

Sanchez, Ingrid ;

Bergerot, Cyrille ;

Thibault, Helene ;

Cung, Thien Tri ;

Finet, Gerard ;

Argaud, Laurent ;

Revel, Didier ;

Derumeaux, Genevieve ;

Bonnefoy-Cudraz, Eric ;

Elbaz, Meier ;

Piot, Christophe ;

Ovize, Michel ;

Croisille, Pierre .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2012, 59 (24) :2175-2181

[7]

Temporal profile of Src, SSeCKS, and angiogenic factors after focal cerebral ischemia: Correlations with angiogenesis and cerebral edema [J].

Zan, Likun ;

Wu, He ;

Jiang, Jie ;

Zhao, Shiguang ;

Song, Yuejia ;

Teng, Guoxin ;

Li, Heng ;

Jia, Ying ;

Zhou, Min ;

Zhang, Xin ;

Qi, Jiping ;

Wang, Jian .

NEUROCHEMISTRY INTERNATIONAL, 2011, 58 (08) :872-879

[8]

Limb remote ischemic postconditioning protects against focal ischemia in rats.[J].Chuancheng Ren;Zhimin Yan;Dingtai Wei;Xuwen Gao;Xiaoyuan Chen;Heng Zhao.Brain Research.2009,

[9]

Analysis of 1008 Consecutive Patients Aged 15 to 49 With First-Ever Ischemic Stroke The Helsinki Young Stroke Registry [J].