Direct observation of the clockwise light-driven rotation of F0F1-ATP synthase complex

The purified thermophilic bacterium PS3 F1βP10×His-tag is inserted into the F0F1-ATP synthases of chro matophores isolated from photosynthetic bacteria Rhodo spirillum rubrum. The studies of biochemical properties of the hybrid chromatophores show that they have both protons-driving capability and photophosphorylation. The fluorescent actin filaments, as a marker of its orientation by video-microscopic experiment, are connected via Maleimido C3-NTA to the reconstituted β10×His-tag of F0F1-ATP synthases. The clockwise rotation of F0F1-ATP synthases driven by light is observed directly when viewed from the F0 side to F1- This system should be valuable for further studying the coupling property of F0F1-ATP synthase.