基于纳米金自组装膜固定辣根过氧化物酶的生物传感研究

被引：4

作者：

左国防

袁焜

潘素娟

机构：

[1] 甘肃省高校新型分子材料设计与功能省级重点实验室

来源：

化学研究与应用 | 2012年 / 24卷 / 01期

关键词：

自组装膜; 纳米技术; 电子传递; 过氧化氢; 电化学生物传感;

D O I：

暂无

中图分类号：

TP212.3 [生物传感器、医学传感器];

学科分类号：

摘要：

将辣根过氧化物酶(HRP)通过纳米技术和自组装技术固定于电极表面,制得了酶修饰电极。纳米金与HRP形成了静电复合物并高效地保持了HRP的生物活性,以对苯二酚作为电子媒介体,差示脉冲伏安法(DPV)研究生物酶电极测定H2O2的线性范围为5.0×10-6～1.0×10-3 mol/L,检测限为2.5×10-6 mol/L,线性方程为△I=0.34765+4.05553CH2O2(mM)。酶电极的表观米氏常数(Kmapp)为0.0675 mmol/L。实验同时证明该生物酶电极具有良好的稳定性和使用寿命。

引用

页码：84 / 89

页数：6

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