植物抗旱和耐重金属基因工程研究进展

被引：15

作者：

张玉秀 ^{[1
]}

徐进 ^{[2
]}

王校 ^{[1
]}

柴团耀 ^{[2
]}

机构：

[1] 中国矿业大学北京化学与环境工程学院生物工程系

[2] 中国科学院研究生院生物系

来源：

应用生态学报 | 2007年 / 07期

关键词：

干旱; 重金属; LEA蛋白; 水孔蛋白; 金属转运蛋白; 草坪草;

D O I：

10.13287/j.1001-9332.2007.0272

中图分类号：

X173 [环境植物学];

学科分类号：

摘要：

干旱和重金属污染严重影响植物的生长发育.植物耐逆相关基因的克隆和功能鉴定研究,为通过基因工程途径提高植物的抗逆性奠定了理论基础.水分亏缺、高盐、低温和重金属胁迫都能诱导LEA(late embryogenesis abundant protein)基因的表达.转基因研究表明,LEA蛋白具有抗旱保护作用、离子结合特性以及抗氧化活性;水孔蛋白存在于细胞膜和液泡膜上,在细胞乃至整个植物体水分吸收和运输过程中发挥重要作用.干旱和盐胁迫促进水孔蛋白基因转录物的积累.过量表达水孔蛋白可增强水分吸收和运输,提高植物的抗旱能力.金属转运蛋白参与重金属离子的吸收、运输和累积等过程.这些蛋白基因在改良草坪草植物的抗旱节水和耐重金属能力等方面具有潜在的应用价值.

引用

页码：1631 / 1639

页数：9

共 9 条

[1] Cloning and expression analysis of SKn-type dehydrin gene from bean in response to heavy metals [J].

Zhang, YX ;

Li, JM ;

Yu, F ;

Cong, L ;

Wang, LY ;

Burkard, G ;

Chai, TY .

MOLECULAR BIOTECHNOLOGY, 2006, 32 (03) :205-217

[2] PCR-enriched cDNA pool method for cloning of gene homologues [J].

Lang, ML ;

Mang, YX ;

Guan, ZQ ;

Chai, TY .

PLANT MOLECULAR BIOLOGY REPORTER, 2005, 23 (03) :219-226

[3] An expression analysis of a gene family encoding plasma membrane aquaporins in response to abiotic stresses in Arabidopsis thaliana [J].

Jang, JY ;

Kim, DG ;

Kim, YO ;

Kim, JS ;

Kang, HS .

PLANT MOLECULAR BIOLOGY, 2004, 54 (05) :713-725

[4]

Transgenic Plants of Colonial Bentgrass from Embryogenic Callus via <Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated Transformation.[J].M.L. Chai;K.K. Senthil;D.H. Kim.Plant Cell; Tissue and Organ Culture.2004, 2

[5] Members of the aquaporin family in the developing pea seed coat include representatives of the PIP, TIP, and NIP subfamilies [J].

Schuurmans, JAMJ ;

van Dongen, JT ;

Rutjens, BPW ;

Boonman, A ;

Pieterse, CMJ ;

Borstlap, AC .

PLANT MOLECULAR BIOLOGY, 2003, 53 (05) :655-667

[6]

Cadmium resistance in transgenic tobacco plants enhanced by expressing bean heavy metal-responsive gene PvSR2.[J].Tuanyao Chai;Qiong Chen;Yuxiu Zhang;Juan Dong;Chengcai An.Science in China Series C: Life Sciences.2003, 6

[7] Characterization of a fertilization-induced and developmentally regulated plasma-membrane aquaporin expressed in reproductive tissues, in the wild potato Solanum chacoense Bitt. [J].

O'Brien, M ;

Bertrand, C ;

Matton, DP .

PLANTA, 2002, 215 (03) :485-493

[8]

Wheat LEA genes; PMA80 and PMA1959; enhance dehydration tolerance of transgenic rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.).[J].Zaiquan Cheng;Jayaprakash Targolli;Xingqi Huang;Ray Wu.Molecular Breeding.2002, 1-2

[9]

Structure and promoter analysis of an ABA- and stress-regulated barley gene; HVA1.[J].Peter F. Straub;Qingxi Shen;Tuan-hua David Ho.Plant Molecular Biology.1994, 2

← 1 →