黄芪甲苷对脑缺血小鼠外周免疫抑制作用的影响

被引：16

作者：

邹莹香 ^{[1
]}

李世春 ^{[2
]}

王志菲 ^{[1
]}

机构：

[1] 上海中医药大学基础医学院

[2] 上海中医药大学中西医结合研究院

来源：

中国中医药信息杂志 | 2021年 / 28卷 / 05期

关键词：

黄芪甲苷; 脑缺血; 外周免疫抑制; 自然杀伤细胞; NKG2D; 小鼠;

D O I：

10.19879/j.cnki.1005-5304.202012268

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的观察黄芪甲苷对脑缺血小鼠外周免疫抑制作用的影响,探讨其保护脑缺血损伤的作用机制。方法实验小鼠随机分为假手术组、模型组和给药组,采用大脑中动脉栓塞法制备脑缺血模型,给药组腹腔注射黄芪甲苷溶液。TTC法检测脑梗死体积,神经功能缺损评分评价小鼠神经功能,观察小鼠脾脏质量变化,流式细胞术检测脾脏和外周血中自然杀伤(NK)细胞比例及NKG2D的表达。结果与假手术组比较,模型组小鼠脑梗死体积增加(P<0.001),神经功能缺损评分明显升高(P<0.001),脾脏质量明显减轻(P<0.001),脾脏和外周血中NK细胞比例明显减少(P<0.05,P<0.001),NKG2D表达明显降低(P<0.001);与模型组比较,给药组小鼠脑梗死体积明显减少(P<0.001),神经功能缺损评分明显降低(P<0.001),脾脏质量明显增加(P<0.05),脾脏和外周血中NK细胞比例明显增加(P<0.05),NKG2D表达明显升高(P<0.001)。结论黄芪甲苷对脑缺血损伤小鼠有明显的保护作用,其机制可能与改善外周免疫抑制作用有关。

引用

页码：55 / 59

页数：5

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