番茄烟粉虱传双生病毒PCR检测

被引:38
作者
何自福
虞皓
罗方芳
机构
[1] 广东省农业科学院植物保护研究所,广东省农业科学院植物保护研究所,广东省农业科学院植物保护研究所广东广州,广东广州,广东广州
基金
广东省自然科学基金;
关键词
烟粉虱传双生病毒; PCR检测; 序列分析;
D O I
暂无
中图分类号
S436.41 [茄果类病虫害];
学科分类号
摘要
From the conserved regions of the reported nucleotide sequences of whitefly-transmitted geminiviruses (WTGV), a pair of degenerate primers was designed to anneal to the conserved sequence. The tomato samples infected geminivirus-like from Guangdong were detected by PCR. The results showed that a 356bp specific fragment was amplified from the samples. The specific fragment was cloned and sequenced, and the sequence was compared with all nucleotide sequences in GenBank by Blast of NCBI. The result showed that the fragment belonged to Geminiviridae DNA. So the degenerate primers may be used to detect the WTGV from tomato in Guangdong. Moreover, both of the homology of the fragment between WTGV from tomato in Guangdong and the reported WTGV in the world and WTGV from tomato in Guangxi were under 82%. These results implied that the WTGV from tomato in Guangdong differed from the above-mentioned WTGV.
引用
收藏
页码:68 / 70
页数:3
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