棕榈藤基因组DNA提取及RAPD反应条件探索

被引：8

作者：

李发根

杨华

尹光天

许煌灿

甘四明

机构：

[1] 中国林业科学研究院热带林业研究所

[2] 中国林业科学研究院热带林业研究所广东广州　

[3] 广东广州　

来源：

林业科学研究 | 2004年 / 06期

基金：

国家科技攻关计划;

关键词：

棕榈藤; 基因组DNA提取; CTAB; RAPD;

D O I：

10.13275/j.cnki.lykxyj.2004.06.023

中图分类号：

S792.91 [棕榈、槟榔];

学科分类号：

摘要：

Genomic DNA was successfully extracted from leaf tissues of two rattan species,Calamus simplificifolius and Daemonorops margaritae,using a modified CTAB method.This method could remove phenolic compounds,polysaccharides and proteins,and the quality and quantity of DNA extracted were reliably characterized.In addition,the reaction composition and amplification program for RAPD analysis were developed with GeneAmp PCR System 2700 (Applied Biosystem Co.).This study provided a technological basis for future molecular studies in rattan and the related species.

引用

页码：824 / 828

页数：5

共 7 条

[1] 尾叶桉和细叶桉无性系的RAPD指纹图谱构建 [J].