免疫亲和柱净化-液相色谱质谱法对粮谷中T-2与HT-2毒素的测定

被引：17

作者：

牟仁祥

曹赵云

金连登

朱智伟

陈铭学

机构：

[1] 中国水稻研究所

来源：

分析测试学报 | 2009年 / 03期

关键词：

T-2毒素; HT-2毒素; 粮谷; 免疫亲和柱; 液相色谱-质谱;

D O I：

暂无

中图分类号：

TS210.7 [产品标准与检验];

学科分类号：

摘要：

建立了免疫亲和柱净化-液相色谱质谱法同时检测粮谷中T-2和HT-2毒素的方法。样品经乙腈-水(体积比80∶20)混合溶剂提取,免疫亲和柱(Bond Elut(Mycotoxin)净化后,采用液相色谱质谱(HPLC-MS)测定。T-2和HT-2毒素在0.005～0.5mg/L范围内线性关系良好,相关系数均大于0.9990;在0.01、0.05、0.10、0.2mg/kg的加标水平下,2种毒素的平均回收率为76%～90%,相对标准偏差为2.8%～7.6%;T-2和HT-2毒素的检出限(S/N=3)分别为0.001、0.002mg/kg。

引用

页码：368 / 371

页数：4

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