玉米细菌性枯萎病及其病原菌的检测技术

被引：5

作者：

吴琼

陈枝楠

范怀忠

金显忠

机构：

[1] 华南农业大学资源与环境学院

[2] 深圳出入境检验检疫局

来源：

中国生物工程杂志 | 2004年 / 12期

关键词：

玉米细菌性枯萎病菌; 检测; 分子生物学; PCR;

D O I：

10.13523/j.cb.20041205

中图分类号：

S435.13 [玉米病虫害];

学科分类号：

摘要：

玉米细菌性枯萎病是影响玉米生产的重要病害 ,该病害通过带菌的种子进行远距离传播 ,对该病菌的检测成为防止和控制该病害的重要手段。介绍现有的检测该病菌的各种方法 ,即黑色素选择培养法、double sandwichELISA以及RAPD PCR ,LCR PCR ,Nested PCR ,multiplePCR和荧光实时PCR等分子生物学检测方法 ,并对这些方法的特性进行比较和探讨。

引用

页码：22 / 25+31 +31

页数：5

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