微滴式数字PCR对肉制品中羊肉和猪肉定量分析

被引：16

作者：

陈晨 ^{[1
]}

张岩 ^{[2
]}

李永波 ^{[2
]}

李鹏 ^{[3
]}

李永艳 ^{[2
]}

张涛 ^{[2
]}

张薇 ^{[2
]}

周巍 ^{[2
]}

张志胜 ^{[1
]}

机构：

[1] 河北农业大学食品科技学院

[2] 河北省食品检验研究院河北省食品安全重点实验室

[3] 部队厨师培训中心

来源：

现代食品科技 | 2018年 / 34卷 / 01期

关键词：

微滴式数字PCR; 肉类掺假; 定量研究; 羊肉; 猪肉;

D O I：

10.13982/j.mfst.1673-9078.2018.1.034

中图分类号：

TS251.7 [产品标准与检验];

学科分类号：

摘要：

为了实现肉制品中羊肉和猪肉的量化研究,本文应用微滴式数字PCR对肉制品进行定量检测。通过在看家基因上设计单拷贝特异性引物,能更好的鉴别是人为因素的掺假还是在加工过程中无意的带入。根据dd PCR的结果显示,在一定范围肉质量和DNA的浓度,DNA浓度和DNA拷贝数(C)成现一定的线性关系。通过人为掺假及市售样品检测以验证此方法。以DNA浓度作为中间换算值,得到肉质量和DNA拷贝数之间的换算关系M_羊=0.12C-10.6 M_猪=0.07C+4。通过对已知掺假比例的肉样及市售样品进行检测,能够较好的得到掺假肉的质量。目前市场上存在一定的掺假现象且该检测方法具有一定市场应用前景,本文建立的微滴式数字PCR在羊肉和猪肉及其制品中的掺假检测具有较强的应用潜力,对目前混乱的肉制品掺假的市场监管提供了科学的依据。

引用

页码：221 / 226+194 +194

页数：7

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