药用植物有效成分生物合成途径解析及其应用

被引：29

作者：

马莹 ^{[1
,2
]}

郭娟 ^{[2
]}

毛亚平 ^{[2
]}

曾雯 ^{[2
]}

詹志来 ^{[2
]}

孟繁蕴 ^{[1
]}

黄璐琦 ^{[2
]}

机构：

[1] 北京师范大学中药资源保护与利用北京市重点实验室

[2] 中国中医科学院中药资源中心道地药材国家重点实验室培育基地

来源：

中华中医药杂志 | 2017年 / 05期

基金：

国家杰出青年科学基金;

关键词：

有效成分; 生物合成; 功能基因; 合成生物学; 代谢工程;

D O I：

暂无

中图分类号：

S567 [药用作物];

学科分类号：

090104 [作物信息科学与技术];

摘要：

药用植物有效成分是中药发挥药效的物质基础,随着分子生物学技术和生物工程技术的快速发展,多学科、多领域方法和技术的联合应用加快了活性成分生物合成的研究,为中药资源的深入挖掘和可持续利用提供了新技术和新策略。依托药用植物的生物合成途径解析,通过药用植物代谢工程改良、合成生物学与发酵工程等手段生产有效成分对缓解中药资源的压力具有非常重要的意义。其中,功能基因的筛选、克隆及研究是进行遗传资源挖掘和可持续利用的基础。文章将从药用植物功能基因研究方法、技术以及应用方面总结目前国内外药用植物功能基因研究思路和进展。

引用

页码：2079 / 2083

页数：5

共 20 条

[1]

利用ISSR和SSR分子标记构建北柴胡遗传图谱 [J].

战晴晴 ;

隋春 ;

魏建和 ;

范圣此 ;

张杰 .

药学学报, 2010, (04) :517-523

[2]

Cytochrome P450 promiscuity leads to a bifurcating biosynthetic pathway for tanshinones[J] Juan Guo;Xiaohui Ma;Yuan Cai;Ying Ma;Zhilai Zhan;Yongjin J. Zhou;Wujun Liu;Mengxin Guan;Jian Yang;Guanghong Cui;Liping Kang;Lei Yang;Ye Shen;Jinfu Tang;Huixin Lin;Xiaojing Ma;Baolong Jin;Zhenming Liu;Reuben J. Peters;Zongbao K. Zhao;Luqi Huang New Phytologist 2016,

[3]

CYP76C1 (Cytochrome P450)-Mediated Linalool Metabolism and the Formation of Volatile and Soluble Linalool Oxides in Arabidopsis Flowers: A Strategy for Defense against Floral Antagonists[J] Boachon Benoi?t;Junker Robert R.;Miesch Laurence;Bassard Jean-Etienne;Hofer Reni?e?;Caillieaudeaux Robin;Seidel Dana E.;Lesot Agni?e?e?s;Heinrich Cli?e?e?e?ment;Ginglinger Jean-Francois;Allouche Lionel;Vincent Bruno;Wahyuni Dinar S.C.;Paetz Christian;Beran Franziska;Miesch Michel;Schneider Bernd;Leiss Kirsten

[4]

A kinetic model for curcumin production in Escherichia coli[J] Daniel Machado;Lígia R. Rodrigues;Isabel Rocha BioSystems 2014,

[5]

Efficient production of flavonoids in Fagopyrum tataricum hairy root cultures with yeast polysaccharide elicitation and medium renewal process[J] Jiang-Lin Zhao;Liang Zou;Cai-Qiong Zhang;Yuan-Yuan Li;Lian-Xin Peng;Da-Bing Xiang;Gang Zhao Pharmacognosy Magazine 2014,

[6]

CYP76AH1 catalyzes turnover of miltiradiene in tanshinones biosynthesis and enables heterologous production of ferruginol in yeasts[J] Juan Guo;Yongjin J. Zhou;Matthew L. Hillwig;Ye Shen;Lei Yang;Yajun Wang;Xianan Zhang;Wujun Liu;Reuben J. Peters;Xiaoya Chen;Zongbao K. Zhao;Luqi Huang Proceedings of the National Academy of Sciences 2013,

[7]

Induction of stilbene phytoalexins in grapevine ( Vitis vinifera ) and transgenic stilbene synthase-apple plants ( Malus domestica ) by a culture filtrate of Aureobasidium pullulans[J] Susanne Rühmann;Judith Pfeiffer;Philipp Brunner;Iris Szankowski;Thilo C. Fischer;Gert Forkmann;Dieter Treutter Plant Physiology and Biochemistry 2013,

[8]

Cytochrome P450 CYP716A53v2 Catalyzes the Formation of Protopanaxatriol from Protopanaxadiol During Ginsenoside Biosynthesis in Panax Ginseng[J] Jung-Yeon Han;Hwan-Su Hwang;Su-Wan Choi;Hyun-Jung Kim;Yong-Eui Choi Plant and Cell Physiology 2012,

[9]

Systematic silencing of benzylisoquinoline alkaloid biosynthetic genes reveals the major route to papaverine in opium poppy[J] Isabel Desgagné‐Penix;Peter J. Facchini The Plant Journal 2012,

[10]

Triterpene Functional Genomics in Licorice for Identification of CYP72A154 Involved in the Biosynthesis of Glycyrrhizin[C][W][OA][J] Seki; Hikaru;Sawai; Satoru;Ohyama; Kiyoshi;Mizutani; Masaharu;Ohnishi; Toshiyuki;Sudo; Hiroshi;Fukushima; Ery Odette;Akashi; Tomoyoshi;Aoki; Toshio;Saito; Kazuki;Muranaka; Toshiya Plant Cell 2011,

← 1 2 →