鲍曼不动杆菌外膜蛋白A通过Akt/mTOR/p70S6K信号通路引起RAW264.7细胞自噬

被引：6

作者：

安志远 ^{[1
]}

丁文一 ^{[2
]}

郑春明 ^{[1
]}

黄骁舾 ^{[1
]}

机构：

[1] 首都医科大学附属北京朝阳医院医学研究中心

[2] 中国医学科学院北京协和医院检验科

来源：

中国病理生理杂志 | 2018年 / 34卷 / 12期

关键词：

鲍曼不动杆菌; 外膜蛋白A; RAW264. 7细胞; 自噬; Akt/mTOR/p70S6K信号通路;

D O I：

暂无

中图分类号：

R378 [病原细菌];

学科分类号：

100103 [病原生物学];

摘要：

目的:分析鲍曼不动杆菌标准株ATCC 19606(Acinetobacter baumannii ATCC 19606)外膜蛋白A(outer membrane protein A,Omp A)对RAW264. 7细胞的自噬诱导作用。方法:建立Omp A刺激RAW264. 7细胞的模型,通过细胞免疫荧光染色、Western blot和透射电子显微镜检测Omp A对RAW264. 7细胞自噬的影响。结果:Omp A可以引起自噬蛋白LC3B-II表达升高,并抑制Akt/mTOR/p70S6K的磷酸化水平;雷帕霉素可以进一步降低mTOR和p70S6K磷酸化,并提高Omp A引起的LC3B-II表达升高。结论:鲍曼不动杆菌Omp A通过Akt/mTOR/p70S6K信号通路引起RAW264. 7细胞自噬。这为将来进一步研究鲍曼不动杆菌引起自噬的分子机制及找到对抗其感染的新方法提供理论依据。

引用

页码：2267 / 2270+2276 +2276

页数：5

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