共 4 条
一种金属螯合连续棒色谱柱的制备及色谱性能
被引：5
作者：
卫引茂
黄晓冬
耿信笃
机构：
[1] 西北大学现代分离科学研究所
[2] 西北大学现代分离科学研究所 西安
[3] 西安
来源：
关键词：
金属螯合色谱;
连续棒色谱柱;
生物大分子;
D O I：
暂无
中图分类号：
O657.7 [色谱分析];
学科分类号：
070302 ;
081704 ;
摘要：
A procedure for the preparation of continuous rod consisting of poly(glycidyl methacrylate\|co\|ethylene dimethacrylate) for immobilized metal affinity chromatography (IMAC) is presented. When chelating Cu(Ⅱ), Zn(Ⅱ) or Ni(Ⅱ) on it, the rod displayed a property of IMAC and the selectivity for protein separation was different from that obtained from the naked rod. An abnormal increase in retention times of proteins was found under the condition of either very high pH or very low pH on the metal chelated rods.
引用
收藏
页码：1664 / 1666
页数：3
相关论文