Graves病患者肠道菌群多样性分析

被引：9

作者：

孙博文 ^{[1
]}

杨孟雪 ^{[1
,2
]}

刘军 ^{[2
]}

周雪 ^{[1
]}

李飞 ^{[1
]}

杨波 ^{[1
]}

机构：

[1] 遵义医科大学附属医院内分泌科

[2] 复旦大学附属上海市第五人民医院内分泌科

来源：

实用医学杂志 | 2020年 / 36卷 / 06期

关键词：

Graves病; 肠道菌群; 16S rRNA基因测序; Th17/Treg轴; 短链脂肪酸;

D O I：

暂无

中图分类号：

R581 [甲状腺疾病];

学科分类号：

100201 [内科学];

摘要：

目的初步探究Graves病(Graves disease,GD)患者肠道菌群的多样性变化。方法选择遵义医科大学附属医院就诊的初诊Graves病患者(n=18例),同期于体检中心体检的健康人群(n=11例),受试者均排除近1个月内合并感染性疾病、其他自身免疫疾病、应用益生菌及抗生素等。并收集其粪便样本,提取肠道菌群DNA,16S rRNA基因扩增,Illumina平台测序,对测序结果进行物种注释、多样性及物种差异分析。结果测序共测得1 200 665条高质量序列,每个样本平均含有(41 402±6 733)条有效序列。2组间Chao1指数、ACE指数、Shannon指数及Simpson指数没有统计学差异(P> 0.05),但GD组均小于NC组,表明Graves病患者肠道菌群多样性降低。2组间肠道菌群组成和结构存在显著差异,门水平上GD组厚壁菌门相对丰度低于NC组,而放线菌门高于NC组(P <0.05)。属水平上GD组双歧杆菌属、柯林斯氏菌属、Pediococcus、N09、02d06等相对于NC组优势丰度分布,而罗斯氏菌属、小杆菌属、Thermus、Slackia、[Prevotella]等相对丰度低于NC组(P <0.05)。结论通过对Graves病患者肠道菌群的多样性分析,可得出Graves病患者与正常人肠道菌群确有差异,肠道菌群紊乱导致肠道慢性炎症状态及免疫激活可能是Graves病的发病机制之一。

引用

页码：767 / 773

页数：7

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