数字PCR的临床应用和挑战

被引：2

作者：

关明 ^{[1
]}

郭玮 ^{[2
]}

刘维薇 ^{[3
]}

娄加陶 ^{[4
]}

田月如 ^{[1
]}

郭永 ^{[5
]}

机构：

[1] 复旦大学附属华山医院检验科

[2] 复旦大学附属中山医院检验科

[3] 上海市第十人民医院检验科

[4] 上海市胸科医院检验科

[5] 清华大学生物医学工程系

来源：

国际检验医学杂志 | 2019年 / 40卷 / 14期

关键词：

体液标本; 单分子; 性能确认; PCR; 临床实验室; 核酸检测; 病原体检测; 临床使用; 液体活检; 拷贝数变异; 病原微生物; 突变检测;

D O I：

暂无

中图分类号：

R440 [];

学科分类号：

摘要：

<正>关明:随着分子遗传知识的积累,DNA和RNA分子的定量变得越来越重要。人们发明了诸多方法,以便尽可能精确地量化核酸的数量。在序列特异性定量方法中,基于聚合酶链反应(PCR)的技术一直是较为理想的选择。实时定量PCR被认为是生物医学实验室的常规操作,但

引用

页码：1665 / 1669+1673 +1673

页数：6

共 7 条

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