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Applications of Digital PCR for Clinical Microbiology

被引：23
作者：
Kuypers, Jane [1 ]
Jerome, Keith R.
机构：
[1] Univ Washington, Dept Lab Med, Seattle, WA 98195 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2017年 / 55卷 / 06期
关键词：
applications; digital PCR; quantitative PCR; REAL-TIME PCR; QUANTITATIVE DETECTION; COPY NUMBER; DROPLET; DNA; QUANTIFICATION; PERFORMANCE; STANDARDS; UTILITY; JC;
D O I：
10.1128/JCM.00211-17
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Digital PCR (dPCR) is an important new tool for use in the clinical microbiology laboratory. Its advantages over quantitative PCR (qPCR), including absolute quantification without a standard curve, improved precision, improved accuracy in the presence of inhibitors, and more accurate quantitation when amplification efficiency is low, make dPCR the assay of choice for several specimen testing applications. This minireview will discuss the advantages and disadvantages of dPCR compared to qPCR, its applications in clinical microbiology, and considerations for implementation of the method in a clinical laboratory.
引用
收藏
页码：1621 / 1628
页数：8
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