实时荧光定量PCR检测RSV胁迫下抗病、感病水稻中与脱落酸相关基因的差异表达

被引：7

作者：

丁新伦

张孟倩

谢荔岩

林奇英

吴祖建

谢联辉

机构：

[1] 福建农林大学植物病毒研究所

来源：

激光生物学报 | 2008年 / 04期

关键词：

武育粳3号; RSV胁迫; 脱落酸相关基因; 实时荧光定量PCR;

D O I：

暂无

中图分类号：

S435.111.4 [侵（传）染性病害];

学科分类号：

090401 ; 090402 ;

摘要：

采用实时荧光定量PCR方法测定了水稻条纹病毒(Rice stripe virus,RSV)胁迫下抗性不同品种水稻中与脱落酸相关基因的mRNA转录水平变化。结果表明:感病品种武育粳3号中WGP1、OsGASA2、Polcalcin、Os-CBL4、Myb和OsCIPK15基因表达水平均上调,上调比率分别为4.96、5.17、2.01、5.17、12.04和7.84。而抗病品系KT95-418中,OsGASA2和OsCIPK15基因表达水平下调,下调比率分别为1/5.40和1/2.08;Polcalcin和Myb基因表达水平上调,上调比率分别为4.20和3.86;WGP1和OsCBL4表达量变化不明显。这些结果表明,RSV胁迫能诱导脱落酸相关基因表达量的变化,并且在抗病、感病水稻品种中的表达特征不同,从而提示植物激素脱落酸可能调控了RSV胁迫条件下相关基因的表达。

引用

页码：464 / 469

页数：6

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