丛枝菌根真菌培养方法研究进展

被引:14
作者
杨晓红
孙中海
邵菊芳
仝瑞建
机构
[1] 华中农业大学柑桔研究所
[2] 西南农业大学园艺园林学院 武汉西南农业大学园艺园林学院
[3] 重庆
[4] 武汉
基金
中国博士后科学基金;
关键词
最新进展; 评述;
D O I
10.13346/j.mycosystema.2004.03.026
中图分类号
Q949 [植物分类学(系统植物学)];
学科分类号
071001 ;
摘要
文章结合最新研究成果着重从十个不同的层面对丛枝菌根真菌(AM真菌)的培养方法研究做了较为系统的介绍与评述。认为活体宿主植物盆栽培养法是最简单、最容易、也是最可靠的AM真菌培养方法,玻璃珠分室培养可方便地将培养基质与AM真菌分开,能获得纯净的AM真菌菌体,具有其它方法不可替代的作用,AM真菌单孢无菌的分室Ri T-DNA转型胡萝卜根双重离体培养是获得AM真菌纯净菌体和研究AM真菌遗传、生理、生化等特性的理想方法,以此方法为基础,在根室中补充碳源、在菌丝生长室置换培养基、多次收获菌体的转型根改良双重培养法是提高某些AM真菌孢子产量的力荐方法。
引用
收藏
页码:444 / 456
页数:13
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