CO2浓度升高对不同大豆品种根际微生物丰度的影响

被引：6

作者：

于镇华 ^{[1
]}

王艳红 ^{[1
]}

燕楠 ^{[2
]}

李彦生 ^{[1
]}

谢志煌 ^{[1
]}

金剑 ^{[1
]}

机构：

[1] 中国科学院东北地理与农业生态研究所黑土区农业生态重点实验室

[2] 中国水利水电科学研究院

来源：

土壤与作物 | 2017年 / 6卷 / 01期

关键词：

CO2; 细菌; 真菌; 反硝化细菌; 荧光定量PCR;

D O I：

暂无

中图分类号：

S154.3 [土壤微生物学]; S565.1 [大豆];

学科分类号：

071012 ; 0713 ; 0901 ;

摘要：

为明确大气CO2浓度升高对不同大豆品种根际微生物丰度的影响差异,全面解析在高CO2浓度下由微生物参与的土壤生态系统碳转化过程,本研究选取1950年-2003年间东北黑土区推广的4个大豆品种,利用开顶式气候箱（Open Top Chamber,OTC）模拟大气CO2浓度升高至550 ppm,采用荧光定量PCR方法,解析了CO2浓度升高对不同大豆品种根际微生物丰度的影响。结果表明:CO2浓度（p=0.006）和大豆品种（p=0.013）对根际细菌16S rRNA基因拷贝数影响显著,CO2升高对4个大豆品种根际土壤细菌丰度均有促进作用,而CO2浓度和大豆品种的交互作用对根际细菌16S rRNA基因拷贝数影响不显著（p=0.362）;CO2浓度、大豆品种以及两者的交互作用对根际真菌ITS基因拷贝数影响均为极显著（p<0.001）,真菌比细菌对CO2浓度升高的响应更为敏感,与细菌和真菌生长所需的碳氮比和生长模式不同有关;CO2浓度（p=0.169）、大豆品种（p=0.404）以及两者的交互作用（p=0.059）对根际反硝化细菌nir S基因拷贝数影响均不显著。

引用

页码：9 / 16

页数：8

共 21 条

[11]

Impact of wheat straw decomposition on successional patterns of soil microbial community structure[J] . Fabiola Bastian,Lamia Bouziri,Bernard Nicolardot,Lionel Ranjard.Soil Biology and Biochemistry . 2008 (2)

[12]

Local response of bacterial densities and enzyme activities to elevated atmospheric CO 2 and different N supply in the rhizosphere of Phaseolus vulgaris L[J] . Susan Haase,Laurent Philippot,Günter Neumann,Sven Marhan,Ellen Kandeler.Soil Biology and Biochemistry . 2007 (5)

[13] Response of aboveground grassland biomass and soil moisture to moderate long-term CO2 enrichment [J].

Kammann, C ;

Grünhage, L ;

Grüters, U ;

Jänze, S ;

Jäger, HJ .

BASIC AND APPLIED ECOLOGY, 2005, 6 (04) :351-365

[14]

Fifteen years of research examining cultivation of continuous soybean in northeast China: A review[J] . Xiaobing Liu,Stephen J Herbert.Field Crops Research . 2002 (1)

[15]

Productivity and compensatory responses of yellow-poplar trees in elevatedC02. Richard J. Norby,Carla A. Gunderson,Stan D. Wullschleger,E. G. O’Neill,Mary K. McCracken. Nature . 1992

[16] Genetic control of root exudation [J].

Rengel, Z .

PLANT AND SOIL, 2002, 245 (01) :59-70

[17] Influence of an elevated atmospheric CO2 content on soil and rhizosphere bacterial communities beneath Lolium perenne and Trifolium repens under field conditions [J].

Marilley, L ;

Hartwig, UA ;

Aragno, M .

MICROBIAL ECOLOGY, 1999, 38 (01) :39-49

[18]

The measurement of soil fungal:bacterial biomass ratios as an indicator of ecosystem self-regulation in temperate meadow grasslands[J] . Richard D. Bardgett,Erica McAlister.Biology and Fertility of Soils . 1999 (3)

[19]

Re-sorption of organic compounds by roots of Zea mays L. and its consequences in the rhizosphere[J] . D. L. Jones,P. R. Darrah.Plant and Soil . 1993 (1)

[20] EXUDATE MOLECULES INITIATING FUNGAL RESPONSES TO SEEDS AND ROOTS [J].

NELSON, EB .

PLANT AND SOIL, 1990, 129 (01) :61-73

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