微滴式数字PCR绝对定量应用研究进展

被引:9
作者
龚永平
郝中香
陈珍容
颜其贵
机构
[1] 四川农业大学动物医学院
关键词
PCR; DNA; GMO; 绝对定量; 微滴;
D O I
暂无
中图分类号
Q503 [生物化学技术];
学科分类号
摘要
<正>1前言PCR是在核酸聚合酶作用下体外扩增DNA或RNA的实验技术。1985年美国科学家Kary Mullis申请了有关PCR的第一个专利[1],至此PCR发展成为科学研究领域的一项关键和常规技术,这种以PCR技术进行定性分析作为第一代PCR,此PCR也可以进行定量检测,但仅能进行终点检测。为了能够实时观察PCR过程和定量分析
引用
收藏
页码:686 / 690
页数:5
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