抗草甘膦转基因大豆(RRS)对根际土壤细菌数量和多样性的影响

被引：28

作者：

徐广惠

王宏燕

刘佳

机构：

[1] 东北农业大学资源与环境学院

来源：

生态学报 | 2009年 / 29卷 / 08期

基金：

黑龙江省自然科学基金;

关键词：

抗草甘膦转基因大豆; 根际土壤; 细菌多样性; DGGE-cloning;

D O I：

暂无

中图分类号：

S565.1 [大豆];

学科分类号：

摘要：

为深入研究种植抗草甘膦转基因大豆的黑土生态区根际土壤中细菌数量及多样性的变化,试验采用DGGE-cloning测序技术与传统培养相结合的方法,研究了抗草甘膦转基因大豆(RRS)对根际土壤细菌数量以及细菌群落多样性的影响。传统培养试验结果为RRS显著降低了土壤细菌的数量;DGGE图谱分析表明,RRS根际土壤细菌16SrDNA条带数、多样性指数及均匀度指数均要低于其他处理,聚类分析显示RRS带谱与RRS-S和Y-S差异较大,相似性分别为64%和64.4%;DGGE-cloning测序结果表明,在RRS处理中缺失条带1和条带12分别属于Unculturedbacterium和Nitrospira门Nitrospira属,其中条带1与其他切取条带最小遗传距离达0.4,与其他处理相比表现出弱势差异的条带2、4、5和条带11均属于Unculturedbacterium。研究表明,RRS不同程度上降低了根际土壤细菌的数量和细菌群落的多样性,并对根际土壤中Nitrospira属细菌有一定的抑制作用。

引用

页码：4535 / 4541

页数：7

共 18 条

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